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Based on this, the following conclusions were drawn: (1) RFp, DNL, ADNL, and PNL all have strong positive correlations with PGA and PGV, whereas fNL has a negative correlation.
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We thank Roy Parker for RFP DCP2-RFP plasmid.
Subcellular fractionation of RFP-RPGR1 19, RFP-RPGR1 195 or RFP alone was performed with six millior COS7 cells singly tRFPsfected with 3 µg of RFP-RPGR1–19, RFP-RPGRORF15 or RFP alone in pDEST-733 vector.
In this way, we created WT::RFP, A4V::RFP and G37R::RFP constructs along with a vector to express RFP alone.
The plasmid tyra-2p::RFP was constructed to identify the expression pattern of TYRA-2.
The xrep-1::rfp completely rescued the xrep-1 k1007) xrep-1 k1007type, but XREP-1::RFP fluorescence was too weak to be useful for the present experimutantdata not shown).
Expression of Pglr-1::rfp::rab-5(GDP) or Pglr-1::rfp::rab-5 GTP) rab-5 GTPressed or enhalsod the accumulation of GLR-1 in accretionsuppressed mutants, respectively (Fig. 8C).
We thank Dan Chase for the pCL40 (Pdop-1::rfp) vector, and King L. Chow for the KC136 strain.
We also generated Pglr-1::rfp::rab-5 GTP), rab-5 GTPtains rab-5 cDNA whichthe mutation Q78L.
To test this hypothesis, we first constructed the xrep-1::rfp fusion gene and transferred it into the xrep-1 k1007) xrep-1 k1007
Moreover, Pglr-1::rfp::rab-5 GTP), rab-5 GTPed into lin-10 mutants, significantly enhanced the accumulation of GLR-1 (Fig. 8B).
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