Polymerization of MMA was effectively performed in ATRP [34, 35], NMP [36] and RAFT [37, 38].
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Via the instability of the 0 0 1 raft structure, the raft structure gradually turns into a wavy one in the second creep stage before its collapse.
The calculations indicate that the 0 0 1 raft structure is stabilized by a small amount of creep deformation but becomes unstable when the creep strain in the γ phase exceeds the magnitude required to fully relax the lattice mismatch.
These features are programmed into SCNP a priori by means of robust living polymerization techniques including ring-opening metathesis polymerization [10] (ROMP), reversible addition-fragmentation chain-transfer [11] (RAFT), and atom transfer radical polymerization [12] (ATRP).
Using a clustering analysis, we further determined that 16 miRNAs were upregulated (Fig. 4A, raft in red) and 25 miRNAs were downregulated (Fig. 4A, raft in green) in the raft cultures when compared to the expression levels in the corresponding monolayer cultures (P<0.05, t-test).
Importantly, our fluorescence-topographic NSOM imaging demonstrated that GM1/CD59 raft markers distributed and re-distributed at mounds but not depressions of T-cell membrane fluctuations.
An increase in the ratio reflects increased CD44 raft affiliation.
Having observed reduced CD44 raft affiliation in migrating highly invasive cells, we sought to directly manipulate CD44 raft affiliation to test its impact on cell motility.
Overexpression of CD44 palmitoylation-impaired mutants reduced the affiliation of CD44 with rafts, but CD44WT-overexpressing cells also presented decreased CD44 raft affiliation.
Monolayer cultures were fixed at passage 3. Raft cultures were grown for 10 days before fixation.
