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Furthermore, we achieve an additional speed up by the observation that we do not need to actually construct a multiple alignment for each set of m + 1 PPIs, but we can estimate an upper bound on its score.
However, if an estimated score of the multiple alignment between these m + 1 PPIs is lower than the score of the best multiple alignment found so far between all the K input PPIs (K ≤ m), we can ignore this combination of transformations and there is no need to try to extend it.
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For example, if there are 300 PPIs from the STRING predicted functional associations dataset are in score range 150~160, and among these 300 PPIs there are 30 PPIs overlapping with the B2H PPI dataset, then in this score range 150~160 the "overlap PPI number ratio" is 30/300 = 0.1.
In addition, we identified all prescriptions for cardiovascular system (C01 C10), acid-related disorders (A02 (PPIs excluded)) and functional GI disorders (A03) in the year before and after the date of the first LDASA prescription.
We consider PPIs and L2-PPIs as two separate data sources.
In addition to improved performance on inferring human HIV-1 PPIs, the proposed SML structure provides a flexible framework for general computational PPI prediction tasks.
To samples suspected to contain more than 5 mg kg−1, a larger volume of PPIS mixture (2 ml) was added.
Mixed working standard solutions of penicillin-d0-piperidine derivatives (PPWS) and mixed internal standard solutions of the penicillin-d10-piperidine derivatives (PPIS) were prepared from the synthesised standards, matching the specific MRL levels in muscle and kidney tissue and in milk and were mixed in the same proportions as described for the penicillin working solutions.
Working standards for tissue, milk and feed containing mixtures of the penicillin-d0-piperidines and penicillin-d10-piperidines were prepared by adding 250 μl of the specific PPWS and 250 μl of the corresponding PPIS in a 10-ml volumetric flask and making up to the mark with water.
The calibrator was represented by the average expression (mean ΔCT) of the seven non-fed samples (PPIS) dissected 24 hours after emerging [ 28].
This reflects increased utilisation of injectable lansoprazole in recent years, with injectable PPIs overall typically 4.2 to 6.8 fold more expensive (CNY/DDD) than their equivalent oral formulations (Table 2).
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