Each of the loci examined had a variable number of observed alleles: 9 for dnaE, 20 for lap, 11 for rstR, 11 for gmd, 2 for recA, 8 for pgm, 4 for gyrB, 7 for cat, and 5 for chi.
Four genes involved in starch biosynthesis and degradation pathway, including ADP glucose pyrophosphorylase (AGP, AT5G48300), phosphoglucomutase (PGM, AT5G51820), disproportionating enzyme (DPE1, 4-α-gluca-notransferase, AT5G64860), and alpha-glucan phosphorylase 2 (PHS2, AT3G46970), were down-regulated by approximately 2 ~ 3-fold.
PPP, pentose phosphate pathway; G6PD, glucose-6-phosphate dehydrogenase; 6PGD, 6-phosphogluconate dehydrogenase; TKT, transketolase; Taldo1, transaldolase; HK, hexokinase; GLUT, glucose transporters; PFK1, phosphofructokinse-1; PGM, phosphoglyceratemutase; PKM2, pyruvate kinase (PK -M2; LDHA, lactate dehydrogenase A; FBPK -M2ctose-1,6-bisphosphate; PEP, phosphoenoLDHAuvate.
Pt Pd Cu/GNR, the most efficient electrocatalyst obtained, exhibits a mass-specific activity of 0.70 mA μgPGM−1 (PGM = platinum-group metal) and specific activity of 1.30 mA cm−2 at 0.93 ViR-free, better than that of Pt Pd Cu/C at 0.90 ViR-free.
Likewise, a role for Ste20p in carbohydrate metabolic processes is supported by the observation that Pak1 phosphorylates and activates phosphoglucomutase-1 (PGM; Ensembl∶ENSG00000079739) [54].
Y. pestis CO92 pgm− was grown in BHI broth at 28°C until OD600 reached 0.2.
L-413C and P2 vir1 phage stocks were prepared on Y. pestis CO92 pgm− as described previously [50] using different temperatures of incubation, 28 or 37°C, respectively.
Mutants in BMEI1415 (wzm), BMEI1886 (pgm) and BMEI1326 (wa**) were retarded with respect to the parental strain whereas BMEII0899 (manBcore) grew faster (Figure S1).
Here, in concert with these changes, many genes in glycolysis were up-regulated, including SAR2684 (fda), SAR0830 (tpiA), SAR0831 (pgm) and SAR2506 (dpgm).
High-concentration stocks of φA1122 were prepared using attenuated Y. pestis strain CO92 pgm− cultured at 28°C by the low multiplicity of infection method [55].
The burst sizes of φA1122, L-413C, and P2 vir1 for Y. pestis CO92 pgm− were about 57, 115, and 9 PFU, respectively (see the plateaus on the phage growth curves in Fig. 2).
