Sentence examples for 1 pfa from inspiring English sources

In order to exclusively analyze virions on the cell surface, cells were fixed with paraformaldehyde (PFA) leaving the plasma membrane impermeable for antibodies during labeling (Figure 1, PFA; Figure S1).

The removal of E. coli and Enterococcus from CSO was always around or above 3 log units using 2 4 mg L− 1 PFA; with a 20 min contact time in both batch-scale and pre-field experiments.

Both morphant and mutant larval zebrafish were sampled at 120 hpf and fixed in 1.5% glutaraldehyde/1% PFA and 70 mM NaPO4, pH 7.2.

Cells were fixed with 0.5 1% PFA for 5 10 min. After fixation the cells were embedded in Vectashield HardSet Mounting Medium containing DAPI for cell nuclei staining.

After centrifugation for 8 minutes at 350 g, the cells were resuspended in 0.5 mL of PBS/1% PFA and kept on ice until the flow cytometry measurement.

After centrifugation, the cells were resuspended in 0.5 mL of PBS/1% PFA and kept on ice until analysis.

At day 4 of culture, Fb were fixed for 20 minutes in 1% PFA diluted in Phosphate-buffered saline (PBS).

Cells were fixed in 1% PFA diluted in staining buffer and stored at 4 °C until further processing.

For experiments in Fig. 8, cells were fixed in 1% PFA for 10 min and permeabilized for 15 min in 0.1% saponin.

OCT-medium embedded embryos were treated and fixed on cover glasses by washing the sample several times with 1% PFA in 1× PBS.

The samples were then permeabilized with 0.5% SDS in 1× PBS for 10 min and postfixed with 1% PFA in 1× PBS for 10 min.