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Cells were rinsed twice and resuspended in × 1 PBS.
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Clean with 1× PBS.
The samples were then permeabilized with 0.5% SDS in 1× PBS for 10 min and postfixed with 1% PFA in 1× PBS for 10 min.
Final pellets (P100) containing exosomes were resuspended in 1× PBS and saved at −80 °C.
Neurons were washed briefly in ice-cold 1× PBS after removing CS.
The fixative was removed with 3× washes of 1× PBS (Amresco, Solon, OH, USA).
They were then rinsed in water and homogenized in phosphate-buffered saline (1× PBS).
Cells were washed with 1× PBS between each step, except for "blocking-primary antibody step".
Cells were then pelleted and rehydrated with 1× PBS at 37 °C for 15 minutes.
Control group: 1× PBS injection.
d 1× PBS injected as control.
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