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Nonetheless, they protect mice against challenge with wildtype clonal Type 1 parasites completely, or clonal Type 2 parasites robustly.
Immunization with Δrps13 parasites protects mice completely against subsequent challenge with wildtype clonal Type 1 parasites, and robustly protects mice against wildtype clonal Type 2 parasites.
There was no significant difference in the growth rate of fabb/f− clone 1 parasites and WT parasites.
To further investigate the phenotype of the knockout parasites we chose to follow the progression of fabb/f− clone 1 parasites in the liver.
3D7 ∆plasmepsinepsin 1) parasites (Liu et al., 2005) and 3D7 parasites expressing ACPleader-cobA-GFP from a pTEOE plasmid (described above) were synchronized using 5% sorbitol and cultured using gentamycin-free media.
To assay the effect of FabB/F deletion on blood stage development, mice were intravenously (iv) injected with 1 × 10 or 1 × 10 fabb/f− clone 1 parasites and blood stage development was followed over time in comparison with wild-type (WT) parasites (Fig. 4A and B).
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Parasite density was expressed per µl with the assumption that 1 parasite per high-powered field (hpf) equals 500 parasites per µl [10].
For spiking 10 and 1 parasite, the hanging drop method was used.
The limit of detection by thin blood film is 0.01% parasitemia (1 parasite in 10,000 erythrocytes or 1,000,000 parasites per ml blood).
742 parasites were brought back on the animals, & were sent to the chief of entomology of the Army Medical Service in Washington.
In a laboratory study, designed to test absolute detection limits, Plasmodium falciparum-infected blood was diluted with uninfected blood to known parasite concentrations ranging from 500 to 0·1 parasites per microlitre (P/μL).
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