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The electrochemical oxidation of 1-hydroxypyrene (1-OHP) has been studied at a screen-printed carbon electrode (SPCE), by cyclic voltammetry and bulk electrolysis.
The PAHs exposure was detected as urinary 1-hydroxypyrene (1-OHP) levels.
The urine 1-OHP, as a sensitive PAHs-exposure biomarker, was determined by LC-MS/MS as previously described [6].
In particular, we use a sensitive biomarker, the urinary 1-OHP, to assess individual PAHs exposure levels.
A Scatter plot and Pearson correlation analysis were conducted to evaluate the relationship between urinary 1-OHP levels and PAH-DNA adducts.
Exposure variables were dichotomized into a low- and a high-exposure group by the urinary 1-OHP levels median value (natural log transformed: 0.71 µg/g of CR).
The individual characteristics and the effects on sperm PAH-DNA adducts and urinary 1-OHP levels were exhibited in Table 1.
Notably, it is found that the median concentration of CR-adjusted 1-OHP in our study is almost 16-fold higher than those in U.S. populations [6].
Our previous studies among Chinese men have assessed the non-occupational exposure to PAHs by measuring 1-OHP concentrations in urine.
Recently, the urinary metabolite 1-hydroxypyrene (1-OHP) has been extensively characterized and may reflect internal PAHs exposure as a sensitive biomarker [10], [11].
We assessed distributions of 1-OHP for normality.
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