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Volumes between 500 pL and 1 nl were microinjected by applying pressure using a picospritzer (IM 300 Cell micronjector, Narishige).
Microinjections of both vegfa16563 (300 pg), tmem33 (250 pg) and tmem33-gfp (1200 pg) mRNA were performed on single-cell-stage embryos with 1 nl injection volume.
To generate F0 CRISPR mutants we injected 1 nl containing 100 pg rheb guide RNA and 200 ng Cas9 protein (PNA bio, CP01) to 1-cell stage embryos.
It is envisioned that concentrations as low as ∼1000 molecules contained in a sample of 1 nl can be detected in a chip-based assay.
For all microarrays, six replicate features per probe were printed, with approximately 1 nL per feature, using an uncoated 90 μm glass dispenser capillary and eight replicate subarrays were printed per microarray slide.
Here, we developed a novel technique using magneto-nanosensors based on the effect of giant magnetoresistance (GMR) for longitudinal monitoring of total and antigen-specific isotype levels with high precision, using as little as 1 nL of serum.
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Plant Research International, Droevendaalsesteeg 1, NL-6708 PB Wageningen, The Netherlands.
Centre for Biosystems Genomics, Droevendaalsesteeg 1, NL-6708 PB Wageningen, The Netherlands.
Gel elements were ∼1 nl in volume (150 μm in diameter and 25 μm in height), and the amount of covalently immobilized saccharide in the glycoarray was 0.4 1.7 pmol per gel element.
For example, the system EENS P and EENS Q can be calculated as EENS_{P} = sumlimits_{j = 1}^{NL} {EENS_{Pj} }, (6) EENS_{Q} = sumlimits_{j = 1}^{NL} {EENS_{Qj} }, (7 where NL is the number of buses in the system.
Injection drop size 0.5 1 nl.
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