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DNA quantification reactions all contained 1 ng template DNA, as determined by Qubit.
For sample GM03756A, we amplified 1 ng of genomic DNA as template using the MyFi Taq (Bioline).
After conversion, 1 ng of converted DNA sample was PCR amplified by Taq DNA Polymerase and processed for Sanger sequencing.
Cells were cultivated in RPMI1640/10% FCS complemented with 1 ng ml−1 mIL-3 for BaF3 and 32D cells.
Mock degraded samples at 1 ng and 10 ng exhibited >90% reportable SNPs.
Levels of DEP and DEHP were over 1 ng mL−1 in some of the samples.
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The samples were collected after 25 cycles of PCR at a typical concentration of ~1 ng µL−1.
Therefore a new SPE pre-treatment was tested, reducing significantly DBP levels (<1 ng).
A limit of detection of 3.2 × 10−9 mol dm−3 (1 ng cm−3) cisplatin was obtained.
pRL-SV40 (1 ng) was used as an internal control.
Detectable mGSTP1 was defined as >1 ng per plasma sample.
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