Sentence examples for 1 neb from inspiring English sources

PCR-RD products were digested with the enzymes indicated in Table 1 (NEB) prior to ligation with T4 DNA Ligase (Invitrogen) according to manufacturer's instructions.

Briefly, modified 3′ and 5′ adapters (TrueQuant, GenXPro) were successive ligated to small RNA (<200 nt) using T4 RNA Ligase 2 and T4 RNA Ligase 1 (NEB), respectively.

RNA samples were treated with the DNase 1 (NEB, UK) as per the manufacturer's recommended protocol to remove potential genomic DNA carryover.

The sequencing library was prepared following the manufacturer's protocol (Illumina) (Bentley et al. 2008) using NEBNext DNA Sample Prep Master Mix Set 1 (NEB).

Biotinylated cDNA ends were captured by Dynabeads M-270 Streptavidin Beads (Life Technologies) and ligated with T4 DNA Ligase 1 (NEB) to modified adapters (TrueQuant, GenXPro).

The PCR product was treated 5 units of T7 endonuclease 1 (NEB) for 15 min at 37°C and then precipitated by addition of 2.5 volumes of ethanol.

Data from four mutant genes were analyzed: Foxa3 [32], Nebulin (neb) [33], Ercc1 [34] and Fibulin-4 (eFigure [35] (Figure 4), using RNA derived from testes, skeletal muscle, liver and aorta respectively.

The mix was ramped from 65°C to 16°C over one hour, before adding 20U T4 ligase together with 1x NEB buffer 2 and 1x ATP (NEB).

DNAs were digested in 30 μl reactions containing 200 ng of genomic DNA, 1 × NEB buffer 4, 8 u PstI-HF, 8 u MseI, incubated at 37°C for 3 h, then 80°C for 20 min to inactivate the enzymes.

Template was combined with 5 μl 10× PCR buffer (NEB), 1 μl 10 mM dNTPs, 1 μl each of 10 μM primer and 0.25 μl Taq (NEB) and ddH2O to a final volume of 50 μl.

Loading control antibodies were specific for: β-actin (rabbit mAb, #4970; NEB), STAT3 (rabbit, #4904; NEB), p42/44 MAPK (mouse mAb, #4696; NEB), and p38α MAPK (mouse mAb, #9217; NEB).