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Open image in new window Fig. 9 Mucus plugging.

In vitro bioefficiency NP uptake was evaluated by flow cytometry on epithelial models (Caco-2/RevHT29MTX (mucus secreting cells)).

In non-treated control mice, the inner stratified Muc2 mucus layer (labeled s) was as usual observed to separate the bacteria in the outer loose mucus layer (labeled o) from the epithelial cells.

PAO levels ranged from 0 to 0.828 pmol peroxide generated min-1 mg-1 mucus and DAO levels ranged from 0 to 7.0 pmol peroxide generated min-1 mg-1 mucus.

If the production of mucus is reduced (for example, due to goblet cell damage, age or hormonal status), [ 1] mucus distribution over the preocular surface is impaired, leading to poor contact of the tear film with the eye surface and a loss of film stability [ 4]. Figure 1 shows the location and extent of epithelial mucins on the ocular surface in a healthy eye compared with a severe dry eye.

7, 17, 18 Mucus secretion was confirmed by Alcian blue and periodic acid Schiff double staining.

This model nominally describes the properties of the MUC2 mucus network in the large intestine.

19 Mucus thickness and growth during this time did not differ between IL-10−/− and WT mice (figure 3B).

As well as the gut microbiota[ 17], mucus[ 18] and the immune system[ 19, 20], intestinal glutathione is suggested to be important for intestinal barrier function[ 15].

NPA was obtained by suction through both nostrils with a Pennine 6 mucus extractor (Pennine Healthcare, Derby, UK) and mucus specimen trap MST-70000, Pennine Healthcare).

13 32 Mucus organisation is also a result of additional cross-linking and processing, defects in which could also contribute to the identified penetrable mucus.

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