Exact(53)
For some experiments macrophages were infected with either 1 MOI BCG or 1 MOI M. tb H37Rv for indicated times.
From this DC T cell co-culture, DCs were depleted by MACS and T cells were cultured for 48h with peritoneal macrophages that were earlier infected for 24h with either 1 MOI BCG or 1 MOI M. tb H37Rv.
(B) RT-qPCR analysis of Aa20 cells treated as in (A) followed by DENV infection at 1 MOI using DENV-specific primers to quantify viral genomic RNA.
(D) Relative transcript levels of AeCHD7 in Aa20 cells infected with 1 MOI of DENV2 analysed at 1 and 5 dpi.
To investigate whether AeCHD7 is required for efficient DENV replication, we knocked down AeCHD7 transcripts in Aa20 cells and challenged these cells with DENV at 1 MOI for 72 h.
(E H) Huh7.5.1 cells were transfected with the indicated siRNAs and then infected with Jc1 at 1 MOI.
Similar(7)
G.M.G.M. Verjans and G.F. Rimmelzwaan (both Dept of Virology, Erasmus MC) provided HSV-1 (MOI 10) and Influenza virus (H1a, MOI 0.2), respectively.
Opsonized promastigotes were used at 5∶1 MOI and amastigotes at 2∶1 MOI.
Macrophages were infected with either promastigotes at a multiplicity of infection (MOI) of 10∶1 or amastigotes at a 3∶1 MOI.
At the peak of reverse transcription of HIV-1 (MOI = 2), each activated mouse T cell contained approximately 5 copies of early reverse transcription product and 3 copies of late reverse transcription product.
PMN (1×107/ml) were co- incubated with C. pneumoniae at a ratio of 1∶1 (MOI 1), equivalent amounts of heat-killed C. pneumoniae, or with mock infected HEp-2 cell lysates as negative controls.
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