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Together, these data indicate that: 1) MES and SUL are responsible for a significant part of the antidopaminergic effects attributed to THD; 2) THD should produce less cholinergic activation than other neuroleptics; and 3) that nonreceptor-mediated effects at high THD concentrations may mask effects due to receptor blockade.
At the time step t, the distances obtained through the communication between MN and M ANs by CSS system are used to build the distance vector: D Arr mes t = d 1 mes t, d 2 mes t, ⋯, d M mes t T, (1).
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We treated rapeseed plants with 0.24 g ha−1 MES (approximately 1% of MES concentration required to control broadleaf weeds in wheat field) for inducing male sterility.
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Quantification was normalized for HM-1 mES cells and revealed Utf1 expression levels of around 50% in O9 iPS cells.
Relative expression of Nanog was found to be two times higher in O9 iPS than in HM-1 mES cells and more than threefold higher in clone UTF-1.
The cultures were grown in screw-cap tubes (18×150 mm) containing about 10 mL VTAC or AF-6 modifiedodified by elimination of CaCO3 and addition of 400 mg L−1 MES [19] [21].
The control extracellular solution (in mM: NaCl 100 (oocyte) or 160 (BHK), CaCl2 1, MgCl2 1, MES 10, pH adjusted to 8 using NaOH) was lowered to various pH values using HCl 2 M. Intracellular patch solution was composed of (mM): CsCl 145, MgCl2 1, MES 10, BAPTA 10, pH 7.2 (CsOH).
Reduced samples were subjected to electophoresis in NuPAGE® precast 4-12% Bis-Tris polyacrylamide gels in 1× MES running buffer for 35 minutes, at 200 V in an x-Cell SureLock™ Mini Cell.
Seedlings were grown before treatment for five days in liquid medium (half-strength MS medium, 1 g L 1 sucrose, 0.5 g L 1 MES, pH 5.7) under a 16 h light/8 h dark regime at 22°C.
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