CDR-1, INF-1, MEM, SQL, TRV, XSS are internal codes for vulnerabilities types and code "?" indicates unknown or uncategorized type of reported vulnerabilities.
The Aβ(1 42) sample was further diluted with 1X MEM to the desired concentration and supplemented with 2 mM l-glutamine, 100 units/ml penicillin, 100 µg/ml streptomycin (Gibco), 1% MEM non-essential amino acid solution (Gibco), 1% MEM vitamins solution (Gibco), and 1% MEM amino acids solution (Gibco).
Briefly, stably transfected CHO cells were grown in DMEM (7%FCS, 2 mM glutamine, 1× MEM non-essential amino acids, 10 units/ml penicillin and 10 ug/ml streptomycin) and supernatants collected.
Feeder-free mouse R1/E ESC were maintained on gelatin-coated dishes in DMEM (GLUTAMAX high-glucose, Gibco) media supplemented with 15% FBS (Gibco), 0.055 mM β-mercaptoethanol (Gibco), 1× MEM non-essential amino acids (Invitrogen), 5000 u/ml penicillin-streptomycin (Invitrogen) and 16 ng LIF (in house production as previously described [32])/250 ml medium.
Inoculum was then removed from the plates and 1× MEM containing 1% agarose was added to cover each well.
Cells were incubated at 37 °C for 30 min, and overlaid with 3 ml of 1× MEM.
After 3 5 days, ICAs were stained with 1× MEM complete medium containing 1% agarose and 0.06% neutral red stain.
For the ICA, monkey serum samples from blood collected 0 7 days post-vaccination were serially diluted 1 10 in completed 1× MEM.
CRL-1660; American Type Culture Collection) were maintained in 1× MEM supplemented with 10% fetal bovine serum, 5% tryptose phosphate broth, 2 mM L-glutamine, 10 mM HEPES, pH 7.4, and 1× MEM nonessential amino acids (1 100 dilution of nonessential amino acids, no. 11140; Gibco, Carlsbad, CA) (Vero medium).
