In our models 1000 cubic feet (1 MCF) of natural gas is equivalent to a calorific value of 1 million British thermal units (1 M Mbtu) in market pricing.
As shown in Author response image 1, MCF represents the fraction of cells containing alterations.
C. difficile strains were grown on Columbia Agar plates (bioMérieux SA, Marcy l'Etoile, France) and subsequent suspensions were adjusted to an optical density of 1 McF at 900 nm using a bioMérieux ATB 1550 densitometer and streaked on the surface of Brucella Agar plates.
Two approaches were taken: (1) MCF-7 tumors were serially transplanted into selective estrogen receptor modulator (SERM -treated immunocompromiSERM -treatedimmunocompromisedERmiceeatoent.
The tumor-bearing mice were randomized into the following four groups (5 animals per group): Group 1 (MCF-7 cell xenograft), Group 2 (MCF-7 cell xenograft+fish oil diet), Group 3 (MDA-MB-435s cell xenograft), and Group 4 (MDA-MB-435s cell xenografts+fish oil diet).
On day 1, MCF-7 & SKBR-3 (5000 cells) and MDA-MB-231 & T47D (20 000 cells) were seeded into 96-well plates in a volume of 100 μl per well.
The standard of statistical significance was the corrected ratio of hybridization signal intensity between Group 1 (MCF-7 cells) and Group 2 (MDA-MB-231 or MDA-MB-468 cells).
As shown in Table 1, MCF-7DOX cells were nearly 8 times more resistance to Doxorubicin than MCF-7CC cells; however, MCF-7DOX cells showed no resistance to paclitaxel and docetaxel.
Two cell lines were used: (1) MCF-7, an adherent human breast cancer cell line (Brooks et al, 1973) and (2) HB-94, a nonadherent mouse B-lymphoid cell line (Brodsky and Parham, 1982) (ATCC, Rockville, MD, USA).
Beginning at a concentration 1000-fold less than the empirically-derived IC50 for each drug (dose 1), MCF-7TAX-2 and MCF-7EPI cells survived 3-fold increases in drug dose until the maximally tolerated dose was reached or selection was stopped.
NIR NFP was able to detect cell-secreted uPA from human cancer cells (SKBR-3, PANC-1, MCF-7, SKOV-3, MDA-MB-231, PC-3, and HT-1080) expressing various levels of uPA.
