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In the CF mouse intestine several mast cell protease genes were expressed: mast cell protease 1 (mast1), mast cell protease 2 (mast2), mast cell chymase 2 (Cmalsoalso called Mcp10), mast cell carboxypeptidase A3 (Cpand, and mast cell protease-like (Mcptl) [17].
Additional file 1: Mast cells are the main interleukin 17-positive cells in anticitrullinated protein antibody-positive and -negative rheumatoid arthritis and osteoarthritis synovium.
We used confocal microscopy to determine which cells in the synovium express IL-17A and IL-17F, double-staining with CD4, CD8, CD15, CD68, CD163, CD31, von Willebrand factor, peripheral lymph node address in, lymphatic vessel endothelial hyaluronan receptor 1, mast cell tryptase and retinoic acid receptor related orphan receptor γt (RORγt).
The total number of mast cells seeded (in 50 μL mast cell suspension) was found to be 18 988 ± 1 046 mast cells (mean ± SEM; n = 4).
The mast cells were added at a 1 : 4 (mast cell : ASM) ratio to reflect our in vivo observations.
To 4 D IV cultures of myenteric neurons 50 μL (= 18 988 ± 1 046 mast cells, mean ± SEM; see section "histochemistry" for details) of mast cell suspension was added.
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Fig. 2 Mast cell-nerve interactions in the human gut.
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