Man 2 (malign, mischievous) "But I know you're shite".
The proposed system is validated by 112 cases (58 malign, 54 benign).
Cytoplasmic staining of the 17 malign and the three benign cell lines with JFC12T10 ranged from weak to very strong.
Strikingly, Sigma Prestige and 1C10 antibodies did not detect any TKTL1 protein in 15 of 17 malign cell line studied.
Monoclonal antibody 1C10 did not show any specific signals in the three benign and in 15 out of 17 malign cell lines tested.
Performing RT-qPCR with three different primer pairs, we identified two of 17 malign human cell lines (12%) characterized by weak endogenous tktl1 mRNA expression (JAR and U251).
In 2 out of 3 benign and 8 out of 17 malign cell lines tested in the present study, TKTL1 protein was detectable in the cytoplasm with the two antibodies JFC12T10 and SigmaPrestige polyclonal antibody by immunohistochemistry.
17 malign and three benign cell lines were characterized according to their expression of TKTL1 on the protein level with three commercially available anti-TKTL1 antibodies utilizing immunohistochemistry and Western blot, as well as on mRNA level with three published primer pairs for RT-qPCR.
This was based on concentration measurement of serum DNA of 24 normal controls, 36 malign breast cancer patients, whereat 6 patients had a metastasizing disease, and 12 patients with a benign breast cancer applying a silica membrane based protocol (Table 1).
CyD1: Cyclin D1, Figures 2/10 describe 2 out of 10 cases, bold: statistical significance, HPF: microscopic high power fields, F Fletcherr [ 10], M Miettinen [ 11], prob malign: probably malignant Bold: significant probability With regard to epithelioid differentiation, 22 GISTs were P53 positive, of which 12 were localized in the stomach and 10 in the intestine.
