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(a) Larvae. 1 = 1 larvae in 150 μl of extraction buffer, 2 = 3 larvae in 150 μl of extraction buffer, 3 = 6 larvae in 150 μl of extraction buffer, and 4 = 12 larvae in 500 μl of extraction buffer.
Experiment Ca 1 larvae were derived from C. ariakensis stocks originating in the Ariake Sea of Japan but accidentally transferred to the west coast of the US with shipments of C. gigas in the 1970s [22].
There were pairwise comparison between the four species conducted as follows: 1) larvae vs. pupae of M. alternates (Ma larvae vs. Ma pupae); 2) pupae of M. alternates vs. S. populnea (Ma pupae vs. Sp pupae); 3) larvae vs. pupae of S. populnea (Sp pupae vs. Sp pupae); and 4) larvae of M. alternates vs. larvae of S. populnea (Ma larvae vs. Sp larvae).
At least 1 larvae per well was found infected.
Late third instar WT and homozygous dPANK/fbl 1 larvae were collect for analysis.
Total RNA extractions were pooled into four samples representing 1) larvae, 2) pupae, 3) adult bodies minus heads, and 4) adult heads.
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(99/100 larvae from 84/85 patients); only 1 larva was identified as A. pegreffii (10 ).
A total of 232 larvae were injected during the experiment: 55 control larvae injected with water, 60 larvae with the ancestor S. marcescens strain, 60 larvae with evolved S. marcescens strain, and 57 larvae with control S. marcescens strain.
Each concentration was applied on 24 larvae distributed in four replicates (each of 6 larvae).
A maximum larva density of 56 larvae · m−2 was recorded.
One will carry about 200,000 larvae and the other about 1.2 million," explained QUT's Matthew Dunbabin in a news release.
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