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qRT-PCR was carried out according to the manufacturer's instructions (LightCycler; Roche Diagnostics) by using a SYBR Green 1 kit.
qRT-PCR was carried out with one method using a LightCycler FastStart DNA Master SYBR Green 1 Kit (Roche Diagnostics GmbH, Mannheim, Germany).
Forty-eight hours postinfection, 2 rabbits (1 adult and 1 kit) died.
The pellet was gently re-suspended in 55 μl of solution #1 (kit component).
End reparation, base addition and adaptor ligation were performed using Methyl-Seq 1 Kit.
Realtime PCR was performed with a LightCycler 1.5 instrument (Roche) using the LightCycler FastStart DNA MasterPLUSSYBR Green 1 kit.
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Ability of PtNPs to inhibit DNA gyrase was evaluated using E. coli DNA gyrase drug screening kit (Cat No: TG2001G-1 kit) from TopoGen by following manufacturer's instructions.
Rare mutations not typically associated with CRC included AKT1 (4), AKT2 (1), IDH1 (1), KIT (1), MAP2K1 (1), PTEN (2), and GNAS (6).
The Chromolize PAI-1 kit is Biopool's third-generation test kit for PAI-1 activity.
The WST-1 kit was used according to the instruction of the provider.
Cell viability was evaluated with the Cell Proliferation Reagent WST-1 kit following the manufacturer's instructions (Roche, France).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com