One of the highest sequence diversities within diploid species was observed in M. schizocarpa (ITC 0856, ITC 0846) (Figure S1A, B/sequence similarity matrix).
Experiments are performed on ISCAS'85, '89 and ITC'99 benchmark circuits.
Genomic DNA of M. acuminata 'Calcutta4' (ITC 0249), M. balbisiana 'Pisang Klutug Wulung', M. ornata (ITC 0637), M. textilis (ITC 0539) and M. beccarii (ITC 1070) was prepared from nuclei isolated from healthy young leaf tissues according to Zhang et al. [64].
Neo-Sensitabs® sensitivity testing is a standardized agar diffusion method which includes antifungal agents in tablets of 9 mm of diameter with FLC (25 μg), ITC (8 μg), KTC (15 μg) and VOR (1 μg).
In the applied concentration range AMB (MIC: 4 8 µg ml−1) and CSP (MIC: 8 32 µg ml−1) were able to inhibit their growth, but resistances to FLC (MIC: 8– >64 µg ml−1), ITC (MIC: 2– >64 µg ml−1), and TRB (MIC: 16– >64 µg ml−1) were observed, except of C. guilliermondii which proved to be sensitive to all antifungal drugs.
Experimental results on ISCAS'89, ITC'99 and IWLS'05 benchmarks show the flexibility of the proposed method to achieve a balance between temperature and test compression.
Similar situation was observed for M. balbisiana 10852 (ITC 0094) which grouped with Australimusa (see Results and Figure 3).
Moreover, the Australimusa/Callimusa clade contained one accession from the Eumusa section - M. balbisiana 10852 (ITC 0094) (Figure 3).
In this study, 4 ITC cases, 10 micrometastases cases and 36 macrometastases cases were observed by permanent histopathological testing.
ITC experiments: Recombinant MUP was over-expressed and purified as described previously.[ 10] ITC experiments were carried out by using a Microcal VP-ITC unit.
