Sentence examples for 1 incubation from inspiring English sources

These significant factors were optimized later by using a central composite design (CCD), which gave optimum conditions at pH of 1, incubation temperature of 37 °C and ionic strength fixed by sodium chloride at 0.2 M when using pepsin.

In Experiment 1, incubation of granulosa cells in increasing concentrations of NH4Cl reduced cell growth, increased cell protein concentrations and increased the amounts of MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) oxidised and oestradiol and progesterone produced per 105 cells.

The SPSC was amplified after 5-HT treatment at the concentration of 10 μmol/L, while the effect of 5-HT induced SPSC was significantly attenuated in the presence of 30 μmol/L -crebanine (1) incubation (Fig. 5).

1) Incubation of tissue lysate with BoNT-B demonstrated a decrease in VAMPI/II protein.

The background signal of the controls (1, incubation without primary antibody; 2, untransfected cells) was subtracted from the raw data.

As shown in Figure 1, incubation of macrophages with MT-II at a concentration of 0.8  μM did not affect macrophage viability.

These features were attenuated when the explants were incubated with anti-CXCR4 antibody before SDF-1 incubation.

To facilitate DNA protein binding, we mixed the ds-oligo pool with 10 µg nuclear extract in 1× incubation buffer (Affymetrix, Santa Clara, CA, USA or Signosis, Santa Clara, CA, USA) at 15 oC for 30 min before transferred to the center of the Spin Columns.

Following the exposure to artificial solar radiation, plastic pieces were removed from the incubation flasks and 0.8 µm filtered surface seawater from the North Adriatic Sea was added to the incubation water at a ratio of 9 1 (incubation water: 0.8 µm filtered).

Using the purified endoinulinase preparation isolated from Aspergillus niger, at a temperature (60 °C), inulin concentration (150 mg ml−1), incubation time (48 h), pH (6.0) and enzyme dosage (60 U ml−1) produced high yields of inulotrioses (70.3 mM), inulotetraoses (38.8 mM), and inulopentaoses (3.5 mM).

In the present study, pectinase concentration (4.04 15.92 ml kg−1), incubation time (48 191 min) and temperature (31 55 °C) were optimized using response surface methodology (RS M for obtaining increased juice yield with higher anti-diabetic (α-amylase and α-glucosidase inhibition) activity, total phenolics and antioxidant activity.