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The 1000 mg l− 1 IBA along with 1000 mg l− 1 NAA induced the largest number of root (8.700/plantlet).
At 48 72 h after MCAO, irregular round- or oval-shaped EGFP/ionized calcium-binding adapter molecule 1 (Iba 1 -positive cells increased in the transition zone, while many amoeboid-shaped or large-cell-body EGFP/Iba 1 -positivecells were increased in number in the innermostransitionischemia.
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Microglial activation was assessed by quantifying calcium binding adaptor protein 1 (IBA-1).
In addition, expression of vimentin and ionized calcium binding adaptor molecule 1 (Iba-1) was assessed to determine cell activation and purity.
Astrogliosis and microglial activation were quantified by measuring glial fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule 1 (IBA-1), respectively.
Calcium binding adaptor protein 1, IBA-1 is expressed by resting microglia and is upregulated when these cells are activated [27].
For visualization of microglia, midbrain sections were immunostained with an antibody recognizing the microglial marker ionizing calcium-binding adaptor molecule 1 (Iba-1) (1∶1,000; Biocare Medical, Concord, CA) and counterstained with cresyl violet.
For staining ionized calcium binding adaptor molecule 1 (Iba-1), sections were treated with antigen retrieval reagent and then incubated with rabbit anti-Iba-1 antibody (Wako Pure Chemical Industries, Ltd., Osaka, Japan) overnight at 4°C.
The purity of the microglia cultures was assessed by double-immunostaining with microglial special markers anti Ionized calcium binding adaptor molecule 1 (Iba-1, 1∶2500, Wako Chemicals Inc. LA) and glia fibrillary acidic protein (GFAP, 1∶400, Millipore Corporation, Billerica, MA).
After 72 hr exposure to HCVcc, HFµφ and HFAs were fixed, permeabilized and immuno-labelled with mouse anti-HCV core (clone 11-B3, GeneTex, Irvine, CA) or mouse anti-HCV NS3 (Abcam07, AbCambridgeridge, MA) and rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1, Wako Chemicals, Neuss, Germany) in HFµφ or rabbit anti-glial fibrillary acidic protein (GFAP, DAKO, Denmark) for HFAs.
In agreement with our previous quantitative findings [17], both Ionized Calcium Binding Adapter Molecule 1 (Iba-1, microglial marker) and Glial Fibrillary Acidic Protein (GFAP, astrocytic marker) demonstrated increased immunostaining in the ipsilateral dorsal horn of the L5 spinal cord on postoperative days 1 and 3 but returned to basal levels by day 9 (Figure 1B).
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