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The transcription of inducible HSPs is regulated by the master transcription factor heat-shock factor 1 (HSF 1).
In order to investigate any cell stress the expression of heat shock transcription factor 1 (HSF), hypoxia inducible factor 1 (HIF1) and lactate dehydrogenase (LDH) was determined by qRT-PCR.
In order to test for any cell stress response, the expression of three marker genes, heat shock transcription factor 1 (HSF), hypoxia inducible factor 1 (HIF) and lactate dehydrogenase (LDH) was determined by qRT-PCR for DPSCs at day 0 and day 28 ± Raman spectroscopy (Fig. 8).
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The heat shock response is mainly regulated at the level of transcription by Heat Shock transcription Factor 1 (HSF-1) both in mammals and C. elegans [4], [5].
A whole animal knockdown of the stress-induced heat shock factor 1 (hsf-1) was shown to be short-lived (Hsu et al., 2003).
The expression of hsp-16.2 gene, which codes for a chaperon, is regulated by the Heat Shock Factor 1 (HSF-1) and DAF-16.
The heat shock factor 1 (HSF-1) is also essential for lifespan extension facilitated by reduced IIS (Hsu et al., 2003; Morley & Morimoto, 2004).
In concert with the stress response transcription factor, Heat Shock Factor 1 (HSF-1), DAF-16 regulates the formation of toxic protein aggregation, reduces the sensitivity to stress and increases lifespan in C. elegans [ 2, 3].
Downregulation of gei-11 activated the heat shock response (HSR) and heat shock transcription factor 1 (HSF-1 -dependent HSF-1 -dependentosolic cHSF-1 -dependentinductionofing proteostasis (Silva et al., 2013).
Inhibition of Hsp90 function also disrupts the chaperone complex with Heat Shock Factor 1 (HSF-1), causing the activation of HSF-1 and induction of heat shock protein expression (5).
Besides DAF-16, the DAF-2 signalling pathway also affects heat shock factor 1 (HSF-1), a transcription factor regulating several heat shock proteins (HSPs) involved in the heat shock response.
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