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Bacteria were cultured in SB broth to an optical density at 600 nm (OD600) of 1, harvested by centrifugation, and resuspended in 10 mM MgCl2 at 104 to 107 cfu/ml.
Xcc wild type and ΔXacPNP strains were grown at 28°C in Silva Buddenhagen (SB) [5] to OD600 of 1, harvested by centrifugation, and resuspended in 10 mM MgCl2 or 50 mM Tris-HCl pH 8 at a density of 107 cfu/ml.
T. crispa dried stems; Figure 1 (harvested at the Selangor housing area in the Herbarium of University Putra in Malaysia, as voucher specimens: KLU 45568) were crushed, and 100 g of the powder was placed in a conical flask and soaked in 900 mL of 95% ethanol for 3 d at room temperature (30 ± 2°C).
Fig. 1 Harvested area under palm oil and the main agricultural crops in Indonesia.
Fig. 1 Harvested volume and carbon balance of ten 10-year periods when net present value (Max NPV), carbon balance (Max C) or discounted carbon balance (Max C, 1%/3% discounting) is maximized.
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In D about 1400 m3∙ha−1 are harvested over two rotations as compared to about 700 m3∙harvestedested in R. The national level features confirmed these regional findings and pointed to a productivity loss of 12.8 million m3∙year−1 for the Fagus and Picea forests used primarily for wood production, which represents about 25%% of Romanian forests yearly production at country level.
(Forest replanted after harvest. 50 km2 harvested annually between 2001 and 2010).
30,000 wolves were harvested annually out of a population of 200,000 during the 1940s, with 40,000 50,000 harvested during peak years.
The UmSRT1-GFP-expressing strain was grown on maltose medium as described [1], harvested at an absorbance at 600 nm (A600) of 0.65, washed twice with water and resuspended in 50-mM Na+-phosphate buffer pH 5.5 (unless otherwise indicated).
Activated T cells were passage 7 days (detailed in Table S4A), harvested and counted.
Briefly, P. aeruginosa cells were grown to OD600nm = 3, harvested, and washed in saline.
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