At both E14.5 and E18.5, Nkx2.5, Gata3, and Gremlin mRNA levels in the stomach of Isl1 MCM/Del mice were lower than controls.
As seen in Figure 1A, Gremlin protein expression in the STZ-treated group was about 1.5-fold greater than in the non-diabetic control mice (N).
We identified 9 genes showing large-scale changes in their expression profile: ELAV- like1, methyltransferase like 7A, dolichyl-phosphate mannosyltransferase, laminin subunit beta-1, gremlin 1, BCL6 corepressor-like 1, and three genes of unknown identity.
These results were consistent with RT-qPCR data and suggest that Isl1 regulates expression of Gata3, Gremlin, and Nkx2.5.
Similarly, the second highly expressed gene GREM1 (Gremlin) is also known to be over-expressed in lung cancer (46).
These genes are sorting nexin 12 (Snx12), gremlin 1 (Grem1), actin gamma 1 (Acollectinllectin sub-family member 12 (Coleukaryoticaryotranslationtion elongation factor 2 (Eef2) and matrix metallopeptidase 2 (Mmp2).
Based on these results, we investigated Isl1, Gata3, Gremlin, and Nkx2.5 expression in Isl1 MCM/F mutant and Isl1 F/+s tomachs using WISH.
Grem2 (gremlin 2), an antagonist of BMP signalling, was among the rhythmic genes identified by the microarray study and was shown to oscillate in antiphase to that of the BMP signalling activities.
To reveal the molecular mechanisms by which Isl1 regulates pyloric development, we assessed the relationship between Isl1 and genes that are required for pyloric development, including Bapx1, Barx1, Nkx2.5, Gremlin, Six2, and Gata3.
