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PCR products were separated using an agarose gel and were analyzed by Quantity 1 gel imaging system (Bio-Rad Co., USA).
Each intermediate and the final product 1 gel were analysed by FTIR spectroscopy.
Magnetic measurements were performed on free-powder samples of 1 gel on a S700X SQUID magnetometer.
EDX measurement confirmed a homogeneous distribution of the metals in 1 gel.
The 1.2 mg/ml collagen 1 gel stiffness mimics the lung environment (approximately 150 Pa), while 2.4 mg/ml collagen 1 gel stiffness mimics the soft tissue environment (~800 1,200 Pa) [ 21, 22].
The positive Curie temperature further indicates a ferromagnetic ordering of the Ni2+ and Fe3+ centres in 1 gel.
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Samples were run on 4.5% polyacrylamide (19∶1) gel and visualized using a PhosphorImager.
Samples were loaded in a 4 8% native acrilamide: bisarilamide (30 1) gel (0.5X TBE buffer) and electrophoresed at 150 V in 0.5X TBE buffer.
Supernatants were carefully removed and the pellet was resuspended in 1× gel loading buffer.
Tumor cells were grown air exposed on top of a collagen-1 gel containing human primary dermal fibroblasts.
Afterward, the PCR product was run on a 1% agarose gel and purified with the Vivantis GF-1 Gel DNA Recovery kit (SKU GF-GD-050).
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