The operating system for this scheme was Linux Ubuntu 10.4 x 64 with GCC 4.4.
Table 3 Configuration of the operating systems under study System FreeBSD Linux OpenSolaris Windows Version PC-BSD OpenSUSE 2008.11 XP Pro 7.0 10.3 SP3 Compiler GCC 4.2.1 GCC 4.2.1 GCC 3.4.3 GCC 4.2.3 User interface KDE 4 Gnome 2.20 Gnome 2.22 Native Processes 66 62 79 16 Free RAM (MB) 630 730 370 830 RAM in cache (MB) 70 140 100 70 Swap in use (MB) 0 0 0 80.
In this case, three different mineral particles were chosen, PCC known as precipitated CaCO3, GCC, ground CaCO3 and kaolin all of which are widely used as fillers in fine papers in the industry.
The H5-specific primer set was as follows: H5-1 GCC ATT CCA CAA CAT ACA CCC, and H5-2 TATTATT CTC TAT CCT CCT TTC CAA, with an expected product size of 358 bp (12, 13 ).
The specimen was tested with the primer set for the H5 gene (forward primer H5-1 GCC ATT CCA CAA CAT ACA CCC, reverse primer H5-2 TATTATT CTC TAT CCT CCT TTC CAA), with an expected product size of 358 bp (8).
The codons involved were 94 (gac D94A gcc, n = 7; gac D94G ggc, n = 5) and 90 (gcg A90V gtg, n = 2) (table 2).
The attribute of GCC can be calculated as (5) GCC = 1 1 + c / C, where c is the average GCC of reads mapped to a given transcript in both samples and C is the average GCC of all mapped reads.
So the initial library is described by the following template sequence: 5′ GCC TCT TGT GAG CCT CCT AAC -N49- CAT GCT TAT TCT TGT CTC CC 3′.
Hb Shepherds Bush (HBB c.224G > A) is an unstable hemoglobin molecule resulting from a β 74 GCC to GAC point mutation (Gly to Asp).
In our patient, PCR sequencing showed the β 74 GCC to GAC point mutation (Gly to Asp) mutation specific to Hb Shepherds Bush.
