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DI and GF developed the model, GF and MH carried out an initial analysis of the model, GF developed the infrastructure for the parameter screens, GC carried out the analysis of the model, MH created Figure 1, GC and DI wrote the paper.
The method proceeds exactly as the Sun-cpg for the single-SNP case (i.e. in equation (1) GC is replaced by GC, GP), except now we calculate Pr(I∣ GC, GP), where GC, GP are the genotypes of parents and children at two or more tightly linked candidate SNPs.
Figure 1 GC profiles of saturated hydrocarbons in heating oil over time.
For rolling or overturning: u 2 ≥ 2 ρ s / ρ w - 1 gc μ s cos θ + c / b sin θ C d c 2 / b 2 + C l (2).
The coexistence of SCLC and GC in the presented case might indicate several possibilities: (1) GC may arise from SCLC via epithelial-to-mesenchymal transition, (2) SCLC may arise from GC through phenotypic conversion, and (3) SCLC and GC may have derived from a common neuroendocrine origin.
For direct PCR reactions using HDA 1 (GC clamped) and HDA 2 primers, 50.0 µl reaction mixture consisted of 5.0 µl of 10× PCR buffer containing 25 mmol/l MgCl2 (Promega, USA); 1.0 µl of 10 mM dNTPs containing dATP, dGTP, dCTP and dTTP (Promega, USA); 1.0 µl of each primer [10 mM] (IDT, USA); 0.25 µl of Go Taq DNA polymerase (Promega, USA).
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12932_2007_84_MOESM1_ESM.zip Additional file 1: GC-MS data for Isle of Wight amber.
Figure 1 GC-MS chromatogram after the extraction and derivatization of saliva spiked in concentration of 0.10 μg/L (that of estriol = 2.0 μg/L).
GC-MS analyses reported herein (data set 1) were carried out using a 60 m DB-5 MS column (0.25 mm id, 0.25 μm phase thickness) programmed as follows: Data set 1 GC-MS data for the crude product of hydrolysis dehydration/methylation of sclareolide by oxalylchloride.
The expression systems containing partial soluble PepP were tested for enzymatic function using a novel, specific PepP activity assay based on the release of L-leucine from the substrate LPP (Figure 1). Figure 1 GC-FID chromatograms before and after hydrolysis of LPP by PepP.
10.7554/eLife.12058.008 Figure 5. Guanylate cyclase 1 (GC-1) co-precipitation with rhodopsin from mouse retinal lysate.
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