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(1) Flow through Ni-NTA, (2) wash Ni-NTA, (3) elution Ni-NTA, (4) elution from gel filtration after cleavage with prescission protease.
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Lane 5: Flow-through of the second Ni-NTA affinity column.
This study shed light on removing atrazine from pesticide production wastewater using a pilot-scale UV/O3/ultrasound flow-through system.
Lane 1 soluble cell lysate before purification; lane 2 flow-through; lanes 3 12: eluted fractions from His-trap column under reducing conditions.
Lane 1, crude extract from Saccharomyces cerevisiae expressing TcTrx; 2, flow-through proteins from the Ni-NTA column; 3, wash; 4 9, TcTrx eluted from Ni-NTA column.
Lane 1, crude extract from E. coli expressing ClPDI; 2, flow-through proteins from the Ni-NTA column; 3, wash; 4 7, ClPDI eluted from Ni-NTA column.
Lane 1, crude extract from Saccharomyces cerevisiae expressing TcAAD; 2, flow-through proteins from the Ni-NTA column; 3 9, TcAAD eluted from Ni-NTA column.
Finally, the matched peptides allow the identification of the proteins using databases (e.g. Mascot Server). Figure 2 Flow-through of Current Phospho-proteomic Analysis.
The innovative design incorporates a venturi-based jet aerator to supply atmospheric oxygen without additional energy consumption to a 3D flow-through modified carbon felt (CF) cathode.
Populations were maintained and exposed to steroidal estrogens in 192 flow-through 18-L glass aquaria.
Next, using the aligned TOCSY spectra of C18 flow-through fractions, we conducted PCA and PLS analysis.
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