(A ) Segments from negative-stain EM images of (1) Flag-MAVS CARD and (2) MAVSΔProTM and those from cryoEM images of (3) Flag-MAVS CARD and (4) MAVSΔProTM.
Set of 0 1 flag variables (mathcal {F} = {F_{e}}, e=1,2, ldots, |mathcal {E}_{T}|) to indicate whether the query has been over road segment (e in mathcal {E}_{T}).
Surprisingly, for the OmpA-177 insertion variants (SA-1, FLAG or myc) heat-modifiability is "normal" again (Figure 6).
Recombinant envelope proteins were purified from growth conditioned cell culture media and subjected to immunoaffinity chromatography, using a monoclonal antibody specific for the HSV-1 flag epitope.
VgrG-1 was found in the lysates of all three strains but only in the immunoprecipitate with Tap-1::FLAG (Fig 4B).
Lysates and immunoprecipitates were subjected to SDS PAGE and analyzed for the presence of TseL, Tap-1::His, and Tap-1::FLAG.
Overexpression of the smaller HPT-1::FLAG protein (23 kDa) driven by P tcu-1 was even more efficient, with greater than 10-fold induction over WT.
TseL was detected in the immunoprecipitates with VgrG-1::FLAG only in wild-type V52, but not in the absence of Tap-1 (Fig 4C).
First, we prepared lysates from V52∆ tap-1 cells transformed with empty plasmid, or plasmid allowing expression of Tap-1 with a His-epitope (p tap-1::His) or FLAG-epitope (p tap-1::FLAG).
To test whether Tap-1 also interacts with VgrG-1, we analyzed lysates of V52 with a chromosomal myc-tagged version of VgrG-1 that was transformed with either empty vector, p tap-1::His or p tap-1::FLAG.
