1.FILA: FILA is a top-k querying schemes based on TAG tree routing.
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Type 2 RNP lacked Cav1, FilA and Dyn2 and had only traces of NSF (Fig. 4E).
Western blot analysis showed the presence of Cav1, FilA, Dyn2, NSF and the various RIC subunits, but the absence of clathrin or of the endosomal components EEA1, Rab5 and Rab7 (Fig. 4D).
The composition of Type 2 vesicles, lacking the components of caveolar endocytosis (Cav1, Dyn2 and FilA) suggests that they are derived from Type 1 vesicles, containing these components, by sorting.
The GFP fluorescence per cell was reduced by depletion of Cav1, Dynamin 2 (Dyn2), Filamin A (FilA) and NSF, but not by knockdown of EEA1, Rab5 or Rab7 (Fig. 2B,C).
Taken together, these results indicate that endocytosis of the RNP into cytosolic transport vesicles is dependent on Cav1, Dyn2 and FilA, but independent of clathrin.
In recent years, there are many top-k querying algorithms (e.g., FILA [3], POT [4], PRIM [5], PRIM-c [6]), which consider how to reduce the amount of transmission data, thus reducing energy consumption and prolonging network lifetime.
He credits his new boat, the 60-foot Fila, with saving his life last April.
