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The Montana Grizzlies football team has a successful program within the NCAA D-1 FCS level.
Dilutions into MEM containing 10% (v v−1) FCS were used for assaying.
All experiments were performed with 1% FCS or without FCS.
DCs were then collected and suspended in cold staining buffer (PBS containing 1% FCS, 0.1 mL) in microcentrifuge tubes.
On the day of the binding assay, cells were washed in PBS and equilibrated in MEM containing 1 % FCS.
Cells were resuspended in PBS supplemented with 1% FCS.
Subsequently, the medium was changed to DMEM/Ham's F12 with 1% FCS.
The suspension was filtered and the cells washed twice by EKRB containing 1% FCS.
Samples were stained in PBS (1% FCS) with the appropriate dilution of the relevant mAbs.
Cells were then left quiescent for 48 h in 1% FCS DMEM.
Electroporated cells were incubated for 3 4 hours in culture medium (DMEM, 1% FCS).
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