Sentence examples for 1 epicentre from inspiring English sources

Four 6-base index sequences were used to prepare bar-coded libraries for sample multiplexing (ScriptSeq™ Index PCR Primers (Set 1); Epicentre Biotechnologies, Madison, WI).

Briefly, 3 5 pituitary glands of 5-month-old female hCGβ-TG mice were enzymatically dissociated in PBS containing 1 g/l d-glucose, 2 g/l collagenase, 1 g/l BSA (Sigma Aldrich), and 200 μM ascorbic acid (Merck) and 60×10 U/l DNase 1 (Epicentre, Madison, WI, USA) for 40 min in a shaker at 37 °C, followed by trituration to disperse the fragments.

The vectors were prepared by PCR amplification using pCC1BAC (Epicentre) or p15A vectors as templates, followed by DpnI (NEB) treatment38.

High-molecular-weight eDNA was blunt-ended (Epicentre, End-It), ligated into pWEB-TNC (Epicentre), packaged into lambda phage and transfected into E. coli EC100 (Epicentre).

After ligation, 2 μl of the mix was transformed into 50 μl TransforMax EPI300 (Epicentre) electrocompetent E. coli cells in a 1-mm cuvette (BTX) at 1,300 V using the ECM 399 Electroporation system (BTX).

In 2008, the Iwate-Miyagi Nairiku earthquake struck Iwate prefecture, northern Honshu, MJMA = 7.2 (epicentre 39.0283N,140.88E).

Indeed, there is a second Pi2 epicentre observed at low latitudes centred around OSAK, where Pi2 onset is observed at the same time as the auroral Pi2 onset location at RABB.

The resin was washed thoroughly with 20 mM Tris-HCl pH 8.0, 10 mM NaCl and TBS-Tween, before adding 20 µl of core-RNAP at 1U µl−1 (Epicentre).

The fosmids are supplied in strain EPI300 (Epicentre) and can be isolated using standard MIDI prep kits.

We suggest recombining this ∼3.2 kb fragment into the fosmid backbone pCC1FOS (Epicentre) to stably transform animals with the unc-119 and the recombineered fosmid.

The ligation and transformation steps were carried out as illustrated in Peterson et al. [16] except that the vector used was HindIII-ready pIndigoBAC5 (Epicentre Technologies).