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To apply cholera toxin B subunit (CTB) as an adjuvant, cry j 1 epi and cry j 2 epi were linked and then fused to the CTB gene in tandem to construct a fusion gene, ctb-linker-cry j 1 epi- cry j 2 epi-flag.
A possible mechanism for the enhanced corrosion protection of EPI coatings on the CRS electrode has been proposed as follows: (1) EPI coatings may act as a physical barrier and (2) the redox catalytic properties of the aniline pentamer units in EPI may induce the formation of a passive metal oxide layer on the CRS electrode, as evidenced by SEM and ESCA studies.
To develop a vaccine that can be used in practical application to prevent and treat Japanese cedar pollen allergy, four major T-cell epitopes from the Cry j 1 antigen and six from the Cry j 2 antigen were selected to design cry j 1 epi and cry j 2 epi, DNA constructs encoding artificial polypeptides of the selected epitopes.
The vaccine effectiveness adjusted by child's age, sex, and location and the main caregiver's literacy was 83.9% (95% CI 70.8%–90.8%) for 1 EPI dose and 90.5% (95% CI 79.7%–95.5%) for 2 doses (EPI and SIAs) (Table 3).
The relative weak influence of the octanol water partition coefficient is clearly illustrated (r = 0.18), despite a substantial correlation of the log KOW with the bioaccumulation factors for fish (Fig. 2b, r = 0.81 for the experimental values, r = 0.79 for Method 1 EPI Suite™ values and r = 0.77 for Method 2 EPI Suite™ values (US EPA 2011).
Implant surgery was performed under local anesthesia (2% lidocaine, 1 : 100,000 epi, sometimes in combination with 4% Articaine, 1 : 200,000 epi).
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Implementation of IPT is modelled in one of two ways, reflecting two different delivery strategies: 1) EPI-linked IPT delivered to infants at specific age-based vaccination contacts (EPI-IPTi) and 2) IPT given outside the EPI programme to all infants, regardless of their exact age, as simultaneous mass treatments during the season of highest malaria transmission (sIPT).
First, we synthesized two different types of dual-radiolabeled conjugates, including 1) 111In-2P-EPI-125I (polymericarrierer 2P was radiolabeled with 111In and drug EPI with 125I), and 2) 125I-2P-EPI-111In (polymeric carrier 2P was radiolabeled with 125I and drug EPI with 111In).
Figure 4 EPI volume structure.
Two DSC-MRI sequences for PWI were investigated: 1) echo planar imaging (EPI) and 2) EPI Keyhole (EPIK).
c Log P ow value was obtained from KOW WIN version 1.67, EPI suite (U.S. Environmental Protection Agency).
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