The drugs and their critical concentrations for resistance were as follows: INH, 0.2 μg·mL-1; RIF, 40 μg·mL-1; SM, 4 μg·mL-1; and EMB, 2 μg·mL-1.
1) Embed the programming in commercials.
†Missing data for INH (106 isolates), RIF (102 isolates), EMB (243 isolates), SM (133 isolates).
The measured EMB concentration in the nominal 200 μg L-1 EMB treatment was 99.5 ± 5.2 μg L-1 EMB.
For the 6 genes investigated, a tendency towards differential expression was evident from day 10 or from day 12 (Emb) onwards.
An initial dataset of 250 emb mutants analyzed in our laboratory, including many that have not been cloned, was published 15 years ago [5].
Statistically significant differences were noted from day 8 on for Ank3 (Figure 7B), from day 10 on for Scin (Figure 7D) and from day 12 on for Actn3 (Figure 7A), Anxa9 (Figure 7C), Emb (Figure 7E) and Mpzl2 (Figure 7F).
To generate samples for microarray experiment 2, salmon lice of either strain were subjected to short term (1 and 3 hour) exposures to 200 μg L-1 EMB, a concentration that would result in >95% immotility in S lice after 24 hours but have no effects in PT lice.
In New York, USA, embC had only 2 EMB resistance-associated nonsynonymous, N394D and R738E, in 75 EMB-resistant samples, and 8 EMB resistance-associated amino acid replacements were identified in embA [ 23].
Escherichia coli strain Tuner™ DE3) (EMB, San Diego, CA) was used for expression and XL10-Gold® (Stratagene, La Jolla, CA) for routine cloning.
The HindIII (Usp22, Emb, Lnp, Mtx2, 19qC2, 3qH2 and 11qA5 loci) or EcoRI (Dlk1 locus) sites investigated are indicated on the maps.
