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We identified several contigs that match cardinal sex determination and differentiation genes (e.g. dsx, fru), or have been implicated in JH or ecdysteroid function (e.g. epoxide hydrolase 1, EcR, ftz-f1, usp).
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at optimum conditions some experimental features like wide 5 2000 ng mL−1 ECR, low detection limit (0.43 ng mL−1, S/N = 3 1) and good repeatability and reproducibility (relative standard deviation, <5.5%, n = 12) indicate versatility in successful applicability of present method for real sample analysis.
Characteristics of plasma parameters in N2/Ar ECR plasma are investigated experimentally and numerically.
In the case of O2/Ar ECR plasma, the ion temperature increases as the oxygen gas concentration is increased.
Thus, our results suggest that the GPR56 ECR has unique and multifaceted regulatory functions, providing novel insights into aGPCR roles in neurobiology.
It is considered that the decrease of the ion temperature in N2/Ar ECR plasma is caused by the decrease of the radial electric field.
Table 1 System specific details for SRSN thin film depositions CVD system Si source gas N source gas RF/MW power (W) Film thickness Deposition rate (Å/min) PECVD 5% SiH4/Ar NH3 50 2200-2600 110-130 ICP CVD 30% SiH4/Ar N2 300 2400-3000 26-30 ECR PECVD 30%SiH4/Ar10%0% N2/Ar 500 800-1200 53-60.
It is considered that the increase of the ion temperature in O2/Ar ECR plasma is caused by energy exchange between electrons and ions because the electron temperature considerably increases with increasing of the oxygen concentration.
A 550 bp region of the RIP140 ECR was amplified by genomic PCR and cloned into a TK-luciferase construct.
Noninfected stage 15 heterozygous (EcRQ 50st /CTG, EcR M55fs /CTG), homozygous (EcRQ 50st /EcRQ 50st, EcR M55fs /EcR M55fs ), and transheterozygous (EcRQ 50st /EcR M55fs ) ecdysone receptor mutant embryos were monitored for development to first-instar larvae.
We then tested the susceptibility of stage 15 EcR Q50st homozygous and EcRQ 50st /EcR M55fs transheterozygous mutant embryos to Ecc15 infection.
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