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We counted the numbers of total germ cells, PAR6 positive germ cells and follicles in the section from the largest cross-section through the center of the ovary at 19.5 dpc, 1 dpp and 3 dpp.
In our study, about one third, half and almost all of germ cells expressed PAR6 at 19.5 dpc, 1 dpp and 3 dpp, respectively (Fig. 1I), but the number of PAR6 positive germ cells was constant during these stages.
Primordial follicle assembly starts at 17.5 dpc and continues during the following 4 5 days, with approximately 30% of oocytes enclosed in primordial follicles at 1 dpp (data not shown).
To identify proteins differentially expressed in 13.5 dpc, 16.5 dpc, and 1 dpp mouse ovaries, 2D-PAGE, MALDI-TOF/TOF, and Statistical analysis was carried out as described [41].
In fetal mouse ovaries, meiosis initiates at 13.5 dpc, progresses to pachytene of meiosis I at 16.5 dpc in most oocytes, and reaches and arrests at diplotene at 1 dpp in almost all oocytes [5], [7].
Then, the PCNA-positive oocytes increased rapidly to 54% in 1 dpp ovaries and reached a plateau in 3 dpp ovaries (>92%), at which time primordial follicles start growing.
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In order to investigate the relationship between PAR6 expression and apoptosis of germ cells, we performed immunohistochemistry with the anti-PAR6 antibody or TUNEL at the two adjacent serial sections of new born (1 dpp-3 dpp) mouse ovaries.
In addition to the degradation of glucagon-like peptide 1, DPP-IV can inactivate substance P (2) and bradykinin (3); both substances are known to produce angioedema.
The effect was rapid, since it occurred most dramatically at 1 dppd and was still evident at 5 dppd.
Furthermore, at 170 dpp transmission electron microscopy confirmed this data.
Type D progressively increased from E11.5 to 3 dpp.
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