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In the control group, viral shedding from the respiratory tract started at 1 dpc and continued with high viral genome loads for four days (on average 2.5×105, 1.4×105, 8.7×104 and 3.6×104 GEC per 100 µl of swab fluid for the first four days which relates to infectivity titres of 5.6×103, 2.5×103, 1.4×103 and 5.1×102 TCin50 in 100 µl of swab fluid, respectively; Table 2).
On the contrary, there were two unvaccinated pigs which excreted virus at 1 dpc and all pigs excreted virus after that until death (Table 2).
By contrast, the challenged virus could be detected in two unvaccinated pigs at 1 dpc and all pigs after that until the death of unvaccinated pigs.
The unvaccinated pigs started to show PR-specific clinical symptoms at 1 dpc and died between 4 and 6 dpc, which showed the high virulence of PRV HN1201 variant.
Sections of trachea from rabbits of group (1) which died within 1 dpc showed hyperplasia in the lamina epithelialis and leukocytic infiltration in the lamina propia and submucosa), in addition to marked submucosal edema accompanied with marked congestion of vessels.
Developmental period 1 indicates the comparisons between stages 94 dpc and 1 dpn; Developmental period 2 indicates the comparisons between stages 1 dpc and 28 dpn; Developmental period 3 indicates the comparisons between stages 28 dpc and 188 dpn; 'Up' indicates higher expression values of later stages, 'down' indicates lower expression values at later stages.
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At 19.5 dpc, the offspring were delivered by caesarean section.
Then, the ESC-injected blastocysts were transferred into 2.5 dpc pseudopregnant C57BL/6 females.
In vivo mouse models of moderate, transient, midgestational (12% O2, 48 h, 12.5 dpc) or severe, acute, early-gestational (5.5 7.5% O2, 8 h, 9.5 10.5 dpc) hypoxia were developed.
Thus, EPC lines were established from aorta gonad mesonephros (AGM) of murine 10.5 dpc and 11.5 dpc embryo when endothelial repertoire is completed.
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