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The annual export of dissolved C compounds (14.4 g C m− 2 year− 1) was dominated by CO2 (9.7 vs. 4.7 g C m− 2 year− 1 DOC).

About 93% Pb in the topsoil could be removed at pH 3.0 by the enhanced DOC treatment, which was performed using a 2500 mg L− 1 DOC solution containing abundant Ca2 + and Mg2 + at an 80 1 liquid/soil ratio (v/v) at 40 °C for 60 min.

Additional file 1: (DOC 367 kb).

Supplementary material 1 (DOC 49 kb) Suppl.

Supplementary material 1 (DOC 1950 kb).

Supplementary material 1 (DOC 2961 kb).

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Similar(41)

The following lysis buffers were used: Triton buffer (50 mM TrisHCl, pH 7.5, 150 mM NaCl, 0.2% Triton X100, 1x protease inhibitor cocktail (Roche)), CHAPS buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.15% CHAPS and 1x protease inhibitor cocktail), or SDS buffer (20 mM triethanolamine 0,14 M NaCl, 0,1% DOC, 0,1% SDS, 0,1% triton X100, protease inhibitor cocktail).

This DOC fraction parameter was calibrated so that modelled annual ha−1 DOC flux matched the observed DOC loads for each catchment separately.

The relative bacterial growth rate (RBGR), as measured by 3H-thymidine incorporation, increased in suspensions of 5 mg C l−1 DOC as compared to control suspensions of 0 mg C l−1.

As the ungauged catchments were assigned DOC parameters based on their hydrologic and physiographic characteristics and not on similar DOM pool sizes, the ha−1 DOC flux values differed slightly from those of the gauged catchments (Table 5).

Table 1 Linear mixed-effects model analysis: the effects of topographic location and seasons on soil moisture, DOC, DON, NH4 +, NO3 − concentrations, net N mineralisation, and net N nitrification in O/A horizon Effects Soil moisture (g g−1) DOC (mg C kg−1) DON (mg N kg−1) NH4 + (mg N kg−1) NO3 − (mg N kg−1) Net N mineralisation (mg N kg−1 wk−1) Net N nitrification (mg N kg−1 wk−1) Coeff.

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