Primers were designed for five 5-HT receptor subtypes: Htr1A (5-HT1and Htr7and5-HT2A); Htr2C (5-HT2C); Htr3 (5-HT3); anDDRtr7 (5-HT7); and DDR 2 (DA D2 receptor) and glycerol-3-phosphate dehydrogenase (Table 1).
Therefore, we used changes in pSmad/HA levels to assay the roles of Col9 1, Ddr, Intβ1 and Vegfr signaling in establishing and maintaining collective leader/trailer polarity.
These results are consistent with the hypothesis that Col9-a1, Ddr, and Vegfr form an incoherent feedforward circuit that may modulate cell-matrix adhesion in migrating TVCs (Fig. 5g).
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All the algorithms were implemented in C++ using the OpenCV library and run on a 1.8 GHz Pentium Dual-Core CPU, with 2Gbyte DDR memory.
Evaluation is performed on a cluster composed of 6 similar servers (two Intel Xeon E5520 quad core CPU 2.2GHz, 8GB DDR RAM, Linux-2.6.18.8 OS, Citrix Xen-5.6.0 hypervisor).
The experiment is conducted on several identical servers (2-way quad-core Xeon E5405 CPU's 2GHz, 8GB DDR RAM) and Redhat Enterprise Linux 5.0 at the host OS and guest OS.
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