Evaluation of transcript abundance using real-time PCR was effective to define six time-points when abundance levels of a set of genes representative for the seed filling process varied with respect to their morphology: 18 DAP (torpedo), 20 DAP (bent- cotyledon), 25 DAP (transition bent-embryo fully fills seed) and 30 DAP, 35 DAP and 40 DAP (embryo fully fills the seed).
For the whole genome microarray gene expression profiling, six time points were selected: (i) 18 DAP (torpedo), (ii) 20 DAP (bent-cotyledon), (iii) 25 DAP (transition bent-embryo fully fills seed), and the developmental stages where the embryo fully fills the seed, being (iv) 30 DAP (v) 35 DAP and (vi) 40 DAP.
Based on DAP beliefs of 242 kindergarten teachers, 30 DAP and 30 DIP teachers were selected.
Averaged over years, whole-milled grain increased until 133 DAP.
Expression of γ-kafirin started decreasing after 21 DAP and a very low expression was detected at 28 DAP.
DNA of the 9 DAP embryos, endosperms, and the parental leaves were extracted.
Thirty (15 DAP, 15 DIP) kindergarten teachers attended the training program while 30 (15 DAP, 15 DIP) comparison kindergarten teachers did not.
Differences in the peroxidases isozyme patterns were observed between 232 DAP and 353 DAP for T2, T4 compared to T3 and T5.
There was a positive correlation between sucrose content and the number of roots at 7 DAP in 'Natal briar' and in 'Rosa progress' at 3 and 7 DAP.
There was statistically a non-significant increase in expression up to 14 DAP, thereafter expression increased significantly (at the 5% level) and reached a maximum at 21 DAP.
There was also a positive correlation between root number and total sugar at 0 and 7 DAP in 'Rosa progress' and 7 DAP in 'Natal briar'.
