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When the fourth quarter began Oct. 1, crude oil was trading at around $100 a barrel.
Fig. 1 Crude death rate by major area 1950 2100 (deaths per 1000 inhabitants).
M Protein ladder; 1 crude enzyme (pET21-egls/DE3); 2 purified enzyme (pET 21-egls/DE3).
Lanes: M, molecular weight standard; 1, crude extract; 2, purified LrXynA.
Lane 1, crude extract from Saccharomyces cerevisiae expressing TcAAD; 2, flow-through proteins from the Ni-NTA column; 3 9, TcAAD eluted from Ni-NTA column.
Lane 1, crude extract from Saccharomyces cerevisiae expressing TcTrx; 2, flow-through proteins from the Ni-NTA column; 3, wash; 4 9, TcTrx eluted from Ni-NTA column.
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Eventually, 50 mL water extract was obtained at a concentration of 2 g mL−1 crude drug.
119.2 mg l−1 crude extract was yielded per batch process after triple extraction of the fluidifized foam.
The water extract was diluted separately to obtain concentrations of 1 and 1.5 g mL−1 crude drug for storage at 4 °C.
On DM basis P. cineraria leaves contained 159.2 g kg−1 crude protein (CP), 567.2 g kg−1 neutral detergent fibre (NDF), 360.0 g kg−1 acid detergent fibre (ADF) and 188.6 g kg−1 acid detergent lignin (ADL).
The highest levels of E S ratio (90 AU kg−1 crude protein), temperature (50 °C) and starting pH (7.7) and the lowest level of frames water ratio (0.71) resulted in the highest NR (76%).
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