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For single cell analysis the cultures were incubated for 105 min after dilution in GM2 at which time cultures were concentrated 10 fold and 5 µL were deposited on a polylysine-coated glass slide (Thermo Scientific) and examined using a Xeiss Axioplan microscope equipped with a Kappa ACC 1 condenser, a Zeiss Plan Neofluor 100× objective and a Kappa DX2 HC-FW camera.
A cheap recording studio can readily make do with 1 dynamic and 1 condenser microphone.
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The recordings in the released corpus were done using a Neumann U87 condenser microphone.
For off-gas regeneration, we used a CO2 absorber and a H2O condenser.
Finally, the third scheme uses a separate 4He Joule Thomson stage to cool the 3He condenser.
The approach was validated against experimental data for an automotive R134a condenser.
These include a plate thermal coil CO2 supercritical heater, a CO2 transcritical turbine, a plate recuperator, an air-cooled finned-tube CO2 condenser and a CO2 liquid pump.
The microscope was operating in confocal mode, using a 32× infinity corrected Schwarzschild objective (NA = 0.65) and a matching 32× condenser.
The ECoScreen2 condenser was also associated with significantly higher concentrations of 8-iso PGF2α.
The microscope used was a Nikon TMS inverted microscope outfitted with a 10x/0.25 objective and 40x condenser phase mask.
The cells were observed using a mock darkfield set-up combining a Ph3 condenser annulus with a 20× objective.
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