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Salmonella was detected down to 1 colony forming unit/ml in food products.
Fig. 1 Colony morphology of strain NCU116-1 (andand its form observed by SEM (b).
Colony frequencies ranged in different samples from 1 colony per 3 to 2000 cells plated.
Recovery of colonies was efficient: approximately 1 colony could be isolated from approximately 13 cells deposited (clonal efficiency of 7.7%).
Based on this criterion, only 0.8% of the sampled colonies were potential mosaics (i.e., 1 colony out of 124).
Given that the negative relationship between colony size and growth rate varied in slope across genotypes (Table 1), colony size clearly has distinct genotype-specific effects on colony growth rate.
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Figure 1 Colony-forming efficiency of organic solvent-tolerant mutants from E. coli JA300 on LBGMg agar medium in the absence of an organic solvent (A) and in the presence of cyclohexane (B) or cyclohexane and p -xylene (7 3 vol/vol mixture) (C).
Three different morphs could be identified, one for P. verenae (PVE) and two for P. apicalis (PAP), respectively: PVE Morph 1 (5 colonies), PAP Morph 3 (1 colony) and PAP Morph 4 (2 colonies) (see supplementary Figures S1, S2 and S3).
These methods are more sensitive than conventional culture techniques with detection limits of approximately 1 colony-forming unit/ml.
Only 1 colony-forming unit was detected in each sample, and due to growth failure these strains could not be identified.
Blood cultures are capable of detecting as low as 1 colony-forming unit (CFU) of bacteria or fungi/yeasts per 10 ml blood.
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