Plant biomass 13C signature was measured by carbon isotope analysis (precision of 0.1‰): combustion in an elemental analyzer (EA1110 CHN, Carlo Erba) coupled via a Conflo II interface to an isotope ratio mass spectrometer (Delta S, Finnigan MAT).
Elemental analyses were performed with a LECO-183 CHN analyzer.
The prepared hydrochars were characterized using the following instruments: Fisons instruments EA 1108 CHN analyser (Fison Instrument, Crawley, UK) was used for CHN analysis; samples were ground into fine powder, weighed into tin capsules and placed on the autosampler for analysis.
As shown in Figure 4A, chn was expressed in wild-type hemocytes and enriched relative to whole third instar larvae 7.5 fold.
The total number of genes identified in these cliques within each population network was: 126 (USA), 114 (CHN), 108 (GER), and 95 (SA).
The number of edges satisfying the PCC in each population network was: 15876 (USA), 12769 (CHN), 11664 (GER), and 9025 (SA).
The number of unique Gene Ontology biological processes terms identified in each of the population networks were: 2806 (CHN), 2801 (GER), 2674 (SA), and 2791 (USA).
The numbers of GO biological processes associated with cliques for each population network were: 247 (USA), 235 (GER), 222 (CHN), and 192 (SA), 247 (USA).
The total numbers of pathways identified for genes present in the CCPs for each population were: 6 (USA), 22 (GER), and 25 (CHN).
The pathways were identified using the KEGG database [ 53, 54], and the number of unique pathways identified for the respective populations were: 99 (CHN), 92 (USA), 54 (GER), and 87 (SA).
