The 50s Chico Hamilton Quintets, immensely popular in their day, have received mixed reactions since.
Nonetheless, single gene manipulations have identified candidate genes for aging by extended longevity phenotypes, and in the model system Drosophila melanogaster such genes include the Insulin-like Receptor [4], chico [5], dFOXO [6], Indy [7] and methuselah [8].
Under this condition, S6K phosphorylates IRS1-4/ chico triggering its internalization and subsequent proteasomal degradation [ 16, 17].
For dominantly marked clones (MARCM system), FRT40 chico 1 or FRT40iso and y w hs-flp UAS-GFP; tub-Gal80 FRT40/CyO y + ; tub-Gal4/TM6B flies were used.
As shown in supplementary material Fig. S4, chico salivary gland cells present a homogeneous distribution of both RFP KDEL and Galt::RFP, similar to wild-type glands.
Comparing median and maximal survival showed that flies pSw-S1106-gal4>ch+/; dSir 2RNAi with RU486 (chico heterozygotes + dSir2knockdown) were similar to pSw-S1106-gal4>ch+/; dSir 2RNAi with RU486 flies (only dSir2 knockdown).
In further examination of the pairwise associations, we find that Rheb is not associated with slif or myc and in contrast to the visualization of the pathway is significantly correlated with TSC1, chico and eIF4E.
We confirmed takeout was increased in Indy long-lived mutants by qPCR and found takeout to be increased in Rpd3, chico, and methuselah mutants, single gene mutations that extend life span [ 14- 16].
The dfoxo Δ94 deletion was then combined with several mutations or genetic manipulations that reduce IIS in Drosophila including a dilp2-3,5 triple mutant (Gronke et al., 2010), median neurosecretary cell (mNSC) ablation (Broughton et al., 2005), chico 1 mutants (Bohni et al., 1999), and Lnk Del29 mutants (Slack et al., 2010).
Take 3 oranges, 4 chico fruits and a banana.
