Exact(1)
Experimental groups were divided into the following: CEF infected by RCASBP A), NY315 RSV, or SR-A RSV (experimental group 1); CEF infected by ts NY72-4 RSV and grown either at the permissive or non-permissive temperature (experimental group 2); and CNR cells infected by ts NY72-4 RSV and grown either at the permissive or non-permissive temperature (experimental group 3).
Similar(59)
The Rf values of the isolated compounds ranged from 0.95 to 0.03 in BEA, 0.95 to 0.78 in CEF and 0.93 to 0.83 in EMW (Table 1).
Thus, the major goal of this study was to conduct global gene expression analysis to profile differentially expressed genes in DF-1 CEF cells compared to their primary CEF counterpart using a 44K chicken oligo microarray.
We studied 42 cirrhotic patients (4 for CEF, 16 for TZP and 22 for MEM) and 42 matched controls.
Note that not all 155 samples contributed evaluable data: based on pre-specified filtering criteria, 5 CMV responses, 5 CEF responses and 5 HIV A Gag responses were excluded in the IAVI dataset; 15 CMV responses, 14 CEF responses and 14 Gag responses were excluded in the HVTN dataset.
Furthermore, ELISPOT analysis showed no difference between CD4+ recall responses to the CMV pp65 peptide or the CD8+ CEF peptide in cells cultured with TRAIL or control protein (Fig. 5B).
Immortal DF-1 CEF cells have been growing continuously in culture for a number of years.
These Spike-in sets were mixed with either primary or DF-1 CEF samples and co-hybridized to the arrays.
These results suggest that DF-1 CEF cells may contain cellular systemic alterations to accelerate metabolic energy expenditure.
The immortal DF-1 CEF cell line was grown using the same conditions as for primary CEF cells.
A two color labeling microarray system was used to compare mRNA expression between primary and DF-1 CEF cells.
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