For instance, H3 H4 chaperones chromatin assembly factor 1 (CAF-1), anti-silencing function protein 1 (Asf1p), histone transcription regulator 1 (Hir1p), and regulator of Ty1 transposition protein 106 (Rtt106p) are important for heterochromatin silencing (Enomoto and Berman, 1998; Huang et al., 2005; Huang et al., 2007; Sharp et al., 2001).
Arabidopsis mutants impaired in chromatin-associated factor 1 (CAF-1) constitutively express 20 IR-induced upregulated genes, including CYCB1 1, and mainly DNA HR genes (Table S3-C).
Unlike ASF1a, ASF1b does not physically intact with HIRA [43], [45], but instead forms complexes with chromatin assembly factor 1 (CAF-1) [54].
In S. cerevisiae the mutation of HIR genes does not alleviate silencing but does exacerbate the telomeric and HM silencing defects associated with inactivation of chromatin assembly factor 1 (CAF-1) [14], [15], [15].
We fused the NTAP tag to the N-terminus of the p150 subunit of the human chromatin assembly factor 1 (CAF-1), a three subunit (p150, p60, and p48) complex that performs DNA replication-coupled histone deposition [65].
For chromatin assembly factor 1 (CAF-1), we used 4191-1 (5'-CACAAGGATGAGATTTTYCARGTICARTGG-3') and 4191-4 (5'-ATTCTCGGCCATTTGCCAIACYTGCATDAT-3') for the first PCR and 4191-2 (5'-TGGGATTTGAGCAAAATHGGIGARGARCA-3') and 4191-3 (5'-CACGGAACAAATAACCCAIGGYTCRTTIGG-3') for the nested PCR.
The replication-dependent histone chaperones include Chromatin Assembly Factor 1 (CAF-1) [ 15] and Rtt106 (at least in yeast) [ 16].
The Chromatin Assembly Factor 1 (CAF-1) complex acts as a major regulator of this process; its intermediate (p60) subunit has been recently proposed as a novel proliferation and prognostic marker for several tumors.
The FAS2 protein is the single Arabidopsis homolog of one of the three subunits (Cac1, Cac2 and another WDR protein, Msi1) of chromatin assembly factor 1 (CAF-1) [ 44], which participates in chromatin assembly following DNA replication or repair [ 45].
Interestingly, the 3/4 of the closest genes to ELG1 affect the chromatin assembly factor 1 (CAF-1) (RLF2 (CAC1 )/ YPR018W ; CAC2 / YML102W and CAC2 / YML102C-A ), responsible for histone deposition during DNA replication.
A role for nucleosome occupancy is further suggested by delayed re-silencing of heat stress-activated repeats in mutants with impaired CHROMATIN ASSEMBLY FACTOR 1 (CAF-1) subunits [ 47].
