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Interactions between domains in (1) buffer, (2) with Ca2+, or (3) ethylenediaminetetraacetic acid (EDTA) were studied by differential scanning calorimetry.
The experimental conditions established with this design were: 8.0 mL min− 1 sampling flow rate, 10.0 mL min− 1 elution flow rate and 0.02 mol L− 1 buffer at pH 5.5.
However, the position of the wider Section 1 buffer in the landscape limited the amount of NO3−-N contaminated groundwater that entered from the agricultural fields, and thus could have been designed to be narrower.
A flow injection disposition was designed improving significantly the sampling rate to 60 samples/h using potassium dihydrophosphate 5 · 10− 3 mol L− 1 buffer (pH 6.8) as carrier and flow rate of 1.5 mL min− 1.
We compare structural and magnetic properties of CoPt films sputtered at 900 K on MgO(1 1 0) (with a Pt(1 1 0) buffer layer) and MgO(0 0 1) (with a Pt(0 0 1) buffer layer) substrates.
The labeled cDNA was hybridized to oligonucleotide microarrays in Ambion Slidehyb #1 buffer (Ambion Europe Ltd).
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Fig. 1 Buffer-aided relaying system model.
They were then subjected to biotin stripping by a reductive cleavage and then solubilized (internal) in TNE/TX-100 1% buffer (25 mM Tris-HCl [pH 7,5], 150 mM NaCl, 5 mM EDTA, 1%TX-100) on ice.
The pellet was washed twice with PBS ×1 buffer to remove excess of His-tagged p53 protein.
After two washes, the sample was incubated with 100 μM recombinant His-tagged p53 protein in PBS ×1 buffer for 2 h at RT.
The functionalized diatoms were washed twice with absolute ethanol; the collected pellet was incubated for 10 min at 100 °C and then washed twice with ethanol and PBS (×1) buffer pH 7.4.
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